Composite

Part:BBa_M36555:Design

Designed by: Christian Castaneda, Thai Nguyen, Andrew Rodriguez   Group: Stanford BIOE44 - S11   (2012-12-06)


Ferredoxin-producing gene with iron sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 185
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 103
    Illegal SapI.rc site found at 523


Design Notes

Optimized codons, G/C content, and repeats to 10 base pairs for S. elongatus.


Source

https://parts.igem.org/Part:BBa_J23119; https://parts.igem.org/Part:BBa_B0034; http://www.ncbi.nlm.nih.gov/gene/4260838; https://parts.igem.org/Part:BBa_B0015; https://parts.igem.org/Part:BBa_I716002

References